FITC标记的磷酸化细胞分裂相关蛋白CSTF64抗体
产品名称： FITC标记的磷酸化细胞分裂相关蛋白CSTF64抗体
英文名称： Anti-phospho-CstF64 (Ser83)/FITC
产品编号： HZ-8522R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-phospho-CstF64 (Ser83)/FITC Conjugated antibody
FITC标记的磷酸化细胞分裂相关蛋白CSTF64抗体
| 产品编号 | bs-8522R-FITC |
| 英文名称 | Anti-phospho-CstF64 (Ser83)/FITC |
| 中文名称 | FITC标记的磷酸化细胞分裂相关蛋白CSTF64抗体 |
| 别 名 | p-CstF-64 (Ser83); p-CstF-64 (S83); CstF64 (phospho-Ser83); CstF64 (phospho-S83); CSTF2T; CF1 64 kDa subunit, tau variant; Cleavage stimulation factor 64 kDa subunit, tau variant; CSTF 64 kDa subunit, tau variant; KIAA0689; TauCstF64; CSTF2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human CstF64 around the phosphorylation site of Ser83 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation. Function: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity). Subunit: The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1. Subcellular Location: Nuclear Similarity: Contains 1 RRM (RNA recognition motif) domain. Database links: Entrez Gene: 1478 Human Omim: 300907 Human SwissProt: P33240 Human Unigene: 132370 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
mRNA前体的多腺苷化是需要多个蛋白因子的两步反应。第一步，多聚腺苷酸化底物的内切核切割，需要CstF（裂解刺激因子），由三个不同亚基组成的异三聚体。CSTF 64含有一个RNA结合结构域，负责CSTF的RNA结合活性。CSTF 64在所有体细胞中均有表达，在减数分裂前期和减数分裂中表达，而不是减数分裂生殖细胞。然而，CSTF 64的一个大变种，称为T CSTF 64，在睾丸减数分裂和减数分裂细胞中大量表达，在大脑中的程度较小，并促进多腺苷酸化的生殖细胞模式。编码CSTF 64的基因（指定的CSTF2）映射到X染色体，而T CSTF 64由常染色体基因编码。在B细胞活化过程中CSTF 64浓度的增加从膜结合到分泌形式切换了IgM重链mRNA表达，提示CSTF 64在B细胞分化过程中调节IgM重链表达起着关键作用。