DNMT3A polyclonal antibody
产品名称： DNMT3A polyclonal antibody
英文名称： DNMT3A polyclonal antibody
产品编号： PAB14129
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DNMT3A.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 44-58 of human DNMT3A.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In PBS (0.05% sodium azide, 0.05% proclin 300)
- Storage Instruction:
- Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:100)
Western Blot (1:2000)
Immunoprecipitation (2 ug)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot
- Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.
Whole cell extracts were immunoprecipitated with 2 ug of DNMT3A polyclonal antibody (Cat # PAB14129).
The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (Lane 1 and 2 respectively).
Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A polyclonal antibody (Cat # PAB14129) (diluted 1 : 2,000) (Lane 3).
- Entrez GeneID:
- 1788
- Protein Accession#:
- Q9Y6K1
- Gene Name:
- DNMT3A
- Gene Alias:
- DNMT3A2,M.HsaIIIA
- Gene Description:
- DNA (cytosine-5-)-methyltransferase 3 alpha
- Omim ID:
- 602769
- Gene Ontology:
- Hyperlink
- Gene Summary:
- CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
- Other Designations:
- DNA MTase HsaIIIA,DNA cytosine methyltransferase 3 alpha,DNA cytosine methyltransferase 3A2,DNA methyltransferase HsaIIIA,OTTHUMP00000120005,OTTHUMP00000120006
- Gene Pathway