左右决定因子2(LEFTY2)重组蛋白说明书

Recombinant Left/Right Determination Factor 2 (LEFTY2) 

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Tyr245~Asp356

Tags: N-terminal His-Tag

Tissue Specificity: Uterus. Subcellular Location: Secreted. Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 13.9kDa

Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are asfollows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

 5. Polymerization of the target protein: Dimerization, multimerization etc.

[USAGE]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[IDENTIFICATION]

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用